Trim Quality Start

TrimQualityStart #

[[step]]
    action = "TrimQualityStart"
    min = u8 # minimum quality to keep (in whatever your score is encoded in)
             # either a char like 'A' or a number 0..128 (typical phred score is 33..75)
    target = Read1|Read2|Index1|Index2

Cut bases off the start of a read, if below a threshold quality.

May produce empty reads, filter those with FilterEmptyReads.

Corresponding options in other software #

  • Trimmomatic: LEADING