Filter Empty

FilterEmpty #

[[step]]
    action = "FilterEmpty"
    segment = "read1" # Any of your input segments, or 'All'

Drop the molecule if the read has length 0. (Use after other processing.)

A special case of FilterMinLen.

This is necessary if your modification can produce ’empty' reads - downstream aligners like STAR tend to dislike these in their input.

On segment=‘All’, only filters reads that are empty in all parts. Use multiple FilterEmpty to filter if any part is empty.